Levamisole In-Tank Treatment for AEFW

After basting all my frags this morning, I found that a few on the floor had some medium to small sized flatworms. I would say I found about 5 of them which isn't to alarming, but it tells me there were eggs somewhere that hatched. The frags that had the flatworms were all down in the floor of the tank so I have a few modifications I'm going to do to my treatment tomorrow. 1st I'm going to directly bast all corals. 2nd I'm going to stir up the floor after about an hour into the treatment. I will post my results after that.
 
One other thing I think is we should do is to leave skimmer off, even we leave collection cup out since the levi might be in the foam itself...
 
How many folks who have been treating here have had trouble with the Levamisole effecting smooth skin corals such as echinatas, lokani, granulosa and such? Thanks
 
I have a few smooth skin sps including a Hawkins and if hasnt effected them one bit.

not affacted at all with me...

Nice. Thanks guys. About to take the plunge also. Damn AEFW. Got my Prohibit today and my digital scale yesterday. Due to my vacation in 2 weeks I will wait to start dosing till Sunday. That way I will be back home to begin tx #3. Wish me luck.

I got enough to treat my system 15 times so I may extend it out a bit longer.
 
i relle hope that we can get some positive results with prohibit before i spend 50 bucks on that and it not work to : / anyone raising pigeons i have like 250 pills for pigeons :(
 
Just doing a bit of an experiment. My hypothesis is that Vermisol does not contain 2.36g of Levamisole per pill, based purely on the apparent difference in effect between the pure powder products and the Foye's dewormer tablet product.

1. Procedure.

Using a turkey baster, I applied approximately 10 newtons of force to the bulb, producing a stream from the open end of the implement of approximately 0.9 meters per second near the tip (JOKE). In any case, I blasted off my A. valida for a few seconds, and found that, yes, even after my second treatment with 24 pills of 23.6mg vermisol (for 34g. est. tank volume) the aefw's just laughed. So I recovered one of the little buggers. I placed it in 1/4 cup tank water at 11:00pm. I then added 0.125 (1/8) pill of Vermisol, waited approximately 30 seconds for it to disintegrate and stirred vigorously.

So, 1/8 tablet = 0.0236g/8 or 0.00295g. The total water volume is 1/4 cup, so: 16 cups per gallon, so to figure a quarter cup in gallons, it's 0.25 cups / 16cups/gal = 0.015625 gallons.

So my total dosage in this experiment (going by the initial protocol laid out in the second post of this thread) would be 5/300x0.015625 = 2.6x10^-4 or 0.00026g. for a proper dosage. To figure number of pills (again, for proper dosage) it's 0.00026g./0.0236g./pill = a whopping 0.011 pills. I put in approximately 0.125 of a pill, so the dosage I gave the aefw is approximately 11.4 times greater than the recommended dose.

2. Results.

The friggin' thing is still alive! It's been 41 minutes so far. Still cruzing the glass of the cup.

3. Discussion/Preliminary Findings.

Foye's Vermisol brand pigeon dewormer does not appear to contain 2.36g of Levamisol as advertised on the label of the bottle.
 
Update. At approximately 52 minutes, teh worm appeared to violently convulse once. It then continued moving forward. Pace appears to be faster than before.
 
Update. At approximately 57 minutes, the worm's tail is now held in a position partially enrolled over it's dorsal surface. Forward motion has ceased. The worm is now having intermittent, slight spasms. It occasionally falls to one side or the other, but can still right itself.
 
Update. At approximately 58 minutes the subject began to flop like a trout out of water. At approximately 61 minutes the subject rolled over onto it's dorsal surface (back). Slow rhythmic spasming and intermittent righting and overturning of the body followed until approximately 1:05. At 1:05, the subject resumed forward motion, but with its posterior partially enrolled beneath it's ventral (under) side. Particles of binder are now accumulating on the ventral side.
 
Update. 1:15 -subject on it's side, partially enrolled around an accumulation of small binder particles. Movement very slow. 1:20 - subject lying on it's dorsal side; began doing sit-ups.
 
at approximately 1 hour and 40 minutes nearly all motion has stopped. Subject effectively dead. To simulate the effects of turning on carbon and skimming, I decanted the treatment water and discarded. I added 1/4 cup tank water back to the cup.

In any case, it appears that at 11x the recommended dosage, this product will kill aefw.
 
at approximately 1 hour and 40 minutes nearly all motion has stopped. Subject effectively dead. To simulate the effects of turning on carbon and skimming, I decanted the treatment water and discarded. I added 1/4 cup tank water back to the cup.

In any case, it appears that at 11x the recommended dosage, this product will kill aefw.


While your acount of your experiment had my crying in my coffee laughing, it is excellent information and a well worth while test.

Thanks!:thumbsup:
 
How many folks who have been treating here have had trouble with the Levamisole effecting smooth skin corals such as echinatas, lokani, granulosa and such? Thanks

Cato

I should probably reiterate here that my issue with the more delicate skinned acros did ocurr during my overdose of PROHIBIT. All the pieces that survived the initial overdose did survive the bulk of the following treatments except for a few that were just too severely damaged.

I guess I pointed that out as a caution not to just take the dump the package in the tank approach.

All this collective experimentation is dialing in the dose range for Levamisol. It also identifying Levamisole products that aren't as effective.

Another thing to keep in mind for all of us, like all other dosing in the SW aquariums we keep, NO TWO SYSTEMS ARE IDENTICAL. The sand beds and live rock are so variable it makes it hard to determine volume. It makes it impossible to determine the volume of intersticial space where a AEFW may hide and enjoy little contact with the Levamisole solution we add.

I've noted several testers subtracting water volume for live rock & sand to calculate treatment volume. Let me clarify, I did not do that. I used the "box" volume of each vessle in my system to figure total treatment volume, then dosed at 5 grams PROHIBIT per 300 gallons of system water (Or 1.66 grams/100 gal if you prefer). I think we need to look at this as the starting SAFE dose. If you are not seeing results, up the dosage slightly with each treatment observing you system for stress.

At a minimum, we have proven that systems as a whole can handle prudent Levamisole treatment and virtually all critters survive.

Keep up the good work!:thumbsup:
 
I seem to have had a bad reaction to the vermisol as well.

I would say with certainty

Stay away from the foys. If you are going to try this at all use the purest levimisol you can get.

The next day a few otherwise healthy colonies rtn'd in my qt and the water was cloudy white even this morning. I dosed on wednesday evening. I am very glad I pulled a few of the real nice ones before I dosed but still disappointed in my losses. I think I'll let the tank recover for a while before I attempt to use the prohibit. I may just hold off until some better dosage data and results come out.

I still can't say for sure if the qt even has FW's. I just want a safe regimen that works for peace of mind.

For anyone who used this method with prohibit in there qt tanks only. Were you able to completely eradicate them? 100%
 
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