<a href=showthread.php?s=&postid=7859830#post7859830 target=_blank>Originally posted</a> by Duarte Conceicao
Greetings from the other side of Atlantic!
A little late but here I am to try the probiotic approach to reef keeping.
But after a few emails changed with the people from Probidio I have a few doubts witch I would like to clarify if possible.
Biodigest
The dosage size. I've got a 500 lt aquarium. The answer from Probidio regarding this it that I should dose the entire vial in the aquarium, since they recommend 1 vial to aquariums up to 1000 lt.
I've read a few posts here that try to relate the over dosage of Biodigest with some RTN cases.
So I’m a little confused about this subject. Any recommendations?
Activated Carbon
Most of the people run's carbon in a 24/7 basis as I do it actually without the probiotic products. From Prodibio I’ve got the following answer
"Change your carbon the week when you don't add BIOPTIM"
I change my carbon once a month. Do you guys think that the Probidio answer should be considered?
Storage
Where do you guys store your Probidio products?
Natural Sea Water
Does anyone use the probiotic approach with NSW? I didn't have in the past (the aquarium is 1 year and 3 months old) any case of hairy algae, cyano, diatom or other types.
Reefbooster and other products schedule
Dosing Reefbooster. From Probidio I’ve got the following
"After the water change wait one day or two before adding the products. I think it would be better to add REEF BOOSTER once and wait 48 h after to add the Bio digest and BIOPTIM "
Is anyone doing this? Or are you dosing all the products in the same day.
Can you help me making a schedule for the dosing of the products taking in consideration that I perform a 15 % water change on a week basis.
Days
1 - Water Change
2 -
3 - Biodigest / Bioptim
4
5
6 - ReefBooster
7
8- Water Change
Don't you think that leaving Reefbooster only 2 days in the system without a water change is a little waste? Should I start to do my water changes only 2 times / month or should I add the Reefbooster on the same day that BioDigest / Bioptim?
System Addiction
Does anyone have any idea about the degree of the addiction of an aquarium in this probiotic approach? I don't believe that the bacteria population start to decrease immediately, but in a mid-term timeline something should happen...
Finished my questions 
I'll try to keep a log about this approach and try to make some photos to fulfill it.
Cheers!!
Hi Duarte - Greetings from England. - Nice tank.
The UK market is also not as developed as the US and Germany- and we percieve ourselves to get the sps that Germany and the US leave over

Probably not true...hehe. I also have a 500ltr reef (net)
Some thoughts on your Qs:
1) Digest: A whole vial is fine IME. Others have had problems with ODing with tissue necrosis - but if your dkh is at nsw levels and steady I'm confident there is a low risk that you will experience problems.
2)Carbon: If its used passively - it wont rapidly adsrob the elements in TPIM therefore giving them time to be used by the bacs. Are you using 0.5 ltr of carbon? Monthly change out worked fine for me. If you wanted to be super cautious - you could take the bag of carbon out for 24 hrs. The thing with carbon is to make sure its super low in PO4 by leaving it to stand in RO/DI water for a couple of days before using it and changing the RO/DI water 2-3 times, this will get any PO4 to leach out before going in the tank.
3)Storage: On a shelf. Prodi products dont need refigerating as they are not exposed to O2 until opened, and therefore dont biochemically react.
4)Natural see water: No experience.
5)Booster: I dosed same day as the other 2, but a few hours appart with Booster last. Also switched the skimmer off overnight. Booster is a fantastic product judging from the polip extention after dosing, but go easy until NO3 PO4 are very low.
6) System addiction: Medically, addicition is indicated when the withdrawal of a compound produces abrupt adverse effects in the metobolism, and/or pscyological functioning.
So no

All that will happen is that the environment will gradually revert to to a different ballance. This may or may not result in higher nutrient levels, and if it does - then the consequences flow from that.
However, just like human diet we can withstand not having vitamins and minerals for protracted periods, but will slowly decline. If we arnt dosing reef booster - an alternative source needs to be added.
On dosing rates for 500ltrs suggest: Digest 1 vial every 2 weeks, TPIM 2 vials every 2 weeks, Booster, 1 vial each week, all after any water change.
Cheers
Simon