refractometer

You use distilled water to calibrate the refrac. to zero, that way you have baseline to start out with.
 
They probably want you to calibrate it before use. I use RO/DI water for calibration. In fact i have never used distilled on one ever and they seem to work fine.
 
I use saltwater refracs. and coolant refracs. everyday at home and work. I check the calibration on mine about once a week. Temperature changes can throw them out more than anything else.
 
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