Update on giant carpet antibiotic treatment

carstennh

New member
Hi there:

First, I would like to thank Minh and the many others for their invaluable help during my giant carpet anemone adventure! Also, I would like to apologize for this somewhat lenghty thread, but I think it may be worth your attention.

I have been doing reefing for about ten years now, and I think I have been pretty successful. I have a ten year old reef tank that is thriving, and still has many of the fish and corals I purchased about 10 years ago (including a clown that I purchased the first day). I also am keeping some other aquaria, including a seahorse tank with now a five year old seahorse couple. All this is not to say that I have not had set-backs, but I have been able to overcome them through extensive reasearch and experimentation.

I set out to establish a tank dedicated to giant carpet anemones about a year ago (without having read enough about it). I let the tank mature for several months before adding a beautiful, at the time healthy appearing anemone into the tank. It did fine for about 3 weeks before perishing. Then I recently bought (now, in retropect unhealthy) carpet from a fellow reefer who claims to have had this carpet in his tank for two years. It died 3 days after being introduced into my tank.

I decided these failures are unacceptable. I did more reasearch, and tried Minh's approach. Without going into too much detail my anemonoe got sick, I treated it per Minh's protocol, put it back inot my tank, and it was just hanging on again. I eventually hypothesized that clearly something was wrong in my tank (even tough the clowns are happy, and some other small frags of coral were doing perfectly). I pinned it down to the fact that I would stir the sand somewhat heavily everytime I would put the carpet back to make space. I will now explain how I came to this comclusion.

As you may or may not know there are basically three types of bugs. Gram positive, gram negative, and anerobes. Now this is somewhat oversimplified, but it will suffice for this discussion. Now, I retreated the carpet with cipro, and it did not improve. I added penicillin for better gram positive coverage, and that did not help things either. Only after adding metronidazole (for anerobe coverage) did the carpet bounce back, and now it is doing beautifully (I also added a UV sterilizer to my system).

This is what I am hypothesizing:

When anemones are really sick, they have an overwhelming infection.

When anemones are just hanging on, but not thriving I am sure there are many possible explanations, but I think the primary reason is not the usual suspects (water parameters, othe tank inhabitants, food, light ect). I think the anemone has likely an indolent infection it cannot ward off, but it is not so bad that it is dying. I think the coverage with penicillin, ciprofloxacin, and metronidazole would cover most of these bugs. I know my sample is ONE, but I think this is an interesting hypotheis I would like tested in YOUR tanks if your carpets are not doing well and you are scratching your heads.

IF any of youo in this hobby and has access to a microbiology lab it would be very interesting to actually swab around the mouth or the stomach of sick anemones, culture this, and then use the isolated bugs to test for antibiotic susceptibility it would make some great reasearch. I have looked into this at work (I an an anesthesiologist), but my institution is too large, and there is too much red tape.

To go further, I think probably a large percentage of all coral in our tanks become sick because of bacterial infection, and what I have said about carpet anemone treatment can possibly be applied to all coral.

Thanks for listening, and I look forward to your comments!

Regards,

Carsten

PS: I use a 10 gallon tank with a powerhead, hang-on filter, heater, egg-crate for separating these items form the rest of the tank, and a AI prime light at 100%. I change the water 100% daily, and add 500mg cipro, 250 penicillin, and 500mg metronidazilewhen I set up the tank. I am confident 250 of cipro, 250 penicillin, and 250mg metronidazole would be just fine also. I am not really sure how these break down in an aquarium with/without light, but if someone is interested to run pharmacokinetics on this it would be interesting!
 
Hi Carstennh

I am in the same situation. My green gigantea has gone through cypro treatment bat when returned to DT is just stay hanging. It does not die but neither is in its normal shape

I am thinking in replicate your experience with the three antibiotics you used
 
Hi,

I have removed the nem from DT and placed in a 30 liter HT with a concentration of 12 mg/l of cypro, 6 mg/l of penicilin and 12 mg/litre of metronidazol

Before in DT :



First day in HT:









 
Interesting. Using antibiotics in sequence instead of simultaneously gives bacteria a stepping stone to multi-drug resistance, so you're right that using them at the same time is ideal. Some antibiotics are less effective when used simultaneously however, but using them simultaneously is still your best at killing off multi-drug resistant bacteria.


Good luck with this! I'll be following along.
 
Not in a very good shape today during the first 24 hours of treatment:



The water got cloudy, I think the rock was carring a strong bioload that started to die because of the antibiotics

The gigantea has detached from the rock wich I think is good in order to have a cleaner water and avoid another variable into the equation

Anywaty it was not in a good condition before the daily water change:



After the water change:





Let´s see what we find tomorrow...
 
During today the nem is not reacting good to the treatment. Not any improvement





I am thinking in three possibilities:

1) To late for the nem to recover. It is a "zombi" anemone. It does not die but is continously deflated. Not any treatment will become it healthy

2) The antibiotic cocktel of cypro+peniciline+metronidazol is too much for the animal. Maybe thinking in removing the metronidazol and penicilin in the next water change tonight

3) keep going with the three antibiotics together

I think i will go with option 2, but I do not have too much hope in recovering the nem. In the previos treatment it reacted fine at some hours into treatment and improved day by day
 
IMO, this one is a lost cause. Even if caught early, I estimate there is only a 40-50% success rate for antibiotic treatment of giganteas. This is compared to approximately 80-90% for magnificas. Seems like gigs just don't bounce back from the trauma as easily.
 
The anemone is dying, in fact I think it started to agonize the previous days in DT. Some days it was trying to remain inflated for some hours but eventually always got deflated again. It was like a roller coster

It is time to let it go...



Next time I am thinking in starting the antibiotic treatment just at arrival although the nem does not shown any deflating behaviour.

After treatment for 2 or three weeks do the transfer to QT

This QT will be stablished in advance in the most possible "aseptic" way. I will stablish a biological filter in a canister filter sheeded only with bottled bacteria (like Microbe lift Nite out II or seachem Stability) and cycled only adding ammonium chloride, no live rock or live sand or anything from DT or other organic source.

Then wait for the nem to recover and stabilize for two or three months in QT.

And them do the transfer to DT

This is the less risky scenario I can imagine in order to increase the survival probabilities

Good luck with your nems
 
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