I know this is going to get me flamed....but....believe it or not the bio-load is: 2 Black False Occ's, 3 green Chromis, 1 sand sifting Goby, I Pseudochromis, 1 Pacific Cleaner Shrimp, 6 Blue Leg Hermit Crabs, 2 Margarita Snails, 2 Turbo Snails, 1 Cerith Snail, and 3 Nassarius Snails. I do one 2gal water change every 2 weeks, use PhosBan and ChemiPure in one of the chambers, and have a beta model of the Sapphire skimmer running for the last week. Water params are; NO3-5ppm, Calc-440, Alk-11dKh, pH-8.3, Phosphates-0, Temp-80F. The only supplements I use are Kent Kalkwasser, and B-Ionic 2 part. I also changed the stock pump to a MJ900, and installed a MiniJet 606 with a HydorFlo in the upper left rear corner, and a MiniJet 404 sunk in chamber #1 attatched to a 90 degree elbow and a duckbill....which provides faster flow through the stock skimmer teeth, and increased tank flow. Quite a little project....but a fully stocked Nano can be done!