Winwood
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If you add acetic acid as the food source, the consumed acetic acid is converted to acetyl-CoA which then enters the TCA energy cycle.
If you add ethanol, the bacteria consumes it and first converts it to acetaldehyde, then to acetic acid which then enters the TCA cycle as acetyl-CoA.
This happens because ethanol and acetic acid are normal byproducts of cell metabolism. We humans produce ethanol in our metabolism, but our liver is obviously able to handle the small amount that is produced.
The cool part about this is that most bacteria can metabolize ethanol and vinegar at dilute concentrations (too high and both become lethal which is why ethanol and vinegar are generally aseptic). So there is no need to "seed" the tank with special bacteria (e.g. nitrobacter)
How much is too much? Well, bacteria can grow in 1.5% ethanol. So if you have a 100 gallon tank, 1.5 gallons can be pure ethanol. :spin2:
You're giving me flashbacks to my Biochem classes at Oregon State! LOL! Thank you for the refresher course, I just hope I don't start losing sleep being haunted by those diagrams again!
I guess it's nice to see some real world application for that stuff though.