Italy's Best! Matured SPS Aquariums.

[Roadtoad]

We must not get hung up on the hGH thing. We have to realize that fraction of hGH in any of those mixtures is going to be a miniscule component of the total protein load...way less than.01% those tablets have very little protein in them think micrograms. hGH is a hormone, it is not a steroid (different structure-remember back to bio or organic chemistry). The truth is that there arent any aminoacids in hGH that are over represented as compared to what you would expect from random selection. My guess is that hGH is doing something else or it is mediating the placebo effect.

Sir, dobiamo parlare. che studia? Io sono professore di neurologia e immunologia.

 

[solbby]

<a href=showthread.php?s=&postid=8786214#post8786214 target=_blank>Originally posted</a> by Roadtoad
Dang, this is a long thread-I only made it to page 14 so far! I have a couple of things to say, after reading here and poking around on reefitalia.

First, growth hormone is not a steroid, it is a protein that has anabolic properties.

Second, I thought maybe the answer to the mysterious aminoacid recipe could be found doing a literature search. (unless it was revealed in the last 8 pages that I havent read yet)
It turns out that several glycoproteins are found in coral skeletons. A couple of references found that the skeletons were enriched in aspartic acid and glycine (at a 3.5 to 1 ratio). If we assume that freezing breaks up human growth hormone into individual amino acids (which it doesn't-not even close) there would be 192 total AA per protein molecule, 11 of which would be Aspartic acid and 8 would be glycine. Because there are 20 "normal" amino acids we would have to conclude that these two are not over-represented in hGH.

Supplements of these two amino acids might be better than whey protein that some of the Reefitalia guys are/were using and would be cheaper and more legal than buying growth hormone!

Check out this page, you can find a paper I referenced regarding aspartic acid. My research into the topic has lead me to the same idea as you, .

http://reefcentral.com/forums/showthread.php?s=&threadid=971190&perpage=25&pagenumber=19

BTW I started dosing aspartic acid into my tank as per the papers referenced and at the concentration listed.

 

[jmann]

Since there are many different sizes of cubes in the trays you buy I think the doze was 5ml per 100 gals or 400L so you can measure that amount out. John

 

[jmann]

Solbby can you expand on the doage you are using say per 50 gals of tank water, I don't know what 1.9 mmol is in volume. Thanks John

 

[Sir]

<a href=showthread.php?s=&postid=8791730#post8791730 target=_blank>Originally posted</a> by Roadtoad
We must not get hung up on the hGH thing. We have to realize that fraction of hGH in any of those mixtures is going to be a miniscule component of the total protein load...way less than.01% those tablets have very little protein in them think micrograms. hGH is a hormone, it is not a steroid (different structure-remember back to bio or organic chemistry). The truth is that there arent any aminoacids in hGH that are over represented as compared to what you would expect from random selection. My guess is that hGH is doing something else or it is mediating the placebo effect.

Sir, dobiamo parlare. che studia? Io sono professore di neurologia e immunologia.

Bè, allora ne sai molto più di me

io sono un quasi biologo!

comunque le tue intuizioni sull'ormone sono giuste!e l'effetto che produce non è un effetto placebo...si apprezza una maggiore crescita...
basterebbe integrare solo alcuni amminoacidi per avere quegli effetti...
sopratutto l'acido aspartico , in quanto è direttamente correlato con la calcificazione...quindi una sua aggiunta può realmente aumentare la sintesi proteica, e quindi portare il corallo ad una crescita maggiore.

ancora non lo provo però!

ciao

 

[Robert Patterso]

<a href=showthread.php?s=&postid=8794097#post8794097 target=_blank>Originally posted</a> by Sir
Bè, allora ne sai molto più di me

io sono un quasi biologo!

comunque le tue intuizioni sull'ormone sono giuste!e l'effetto che produce non è un effetto placebo...si apprezza una maggiore crescita...
basterebbe integrare solo alcuni amminoacidi per avere quegli effetti...
sopratutto l'acido aspartico , in quanto è direttamente correlato con la calcificazione...quindi una sua aggiunta può realmente aumentare la sintesi proteica, e quindi portare il corallo ad una crescita maggiore.

ancora non lo provo però!

ciao

Bè, then you know of it a lot more of me

I am an almost biologist!

However your intuitions on the hormone I am just! and the effect that produces is not an effect placebo. ..si appreciates a greater growth... It would suffice to integrate alone some amminoacidi to have those effects... Above all the acid aspartico, as directly is correlated with the calcification. ..therefore its addition really can increase the protein synthesis, and therefore to carry the corallo to a greater growth.

 

[parshmar]

BTW I started dosing aspartic acid into my tank as per the papers referenced and at the concentration listed.

Solby, do you have any results to share with us yet?

 

[Roadtoad]

Solbby, I finally went back and read the rest of the thread. I also found that reference, amazing what pubmed and google scholar will do! What I didnt get from that paper is what protein that it is associated with. The coral biologists really need to get busy and clone that stuff so we cen know. Assuming that the concentration of Asp in NSW is close to 0 then we have to conclude that they concentrate it in their skeletons almost certainly in the form of a protein. Glycine is also present, as are the other aminos That is why I am not sure if dosing Asp alone will have a great effect, do we have any reason to think that Asp is limiting in a tank or in the "glutton"? When you look a this whole method, may be just a really good feeding program, like demonstrated by the paper you cited, fed corals calcify more.

I guess you are doing the Asp experiment, are you dosing at 1.9 mmol/liter right? I dont know if that concentration into the tank water will be an effective dose. It is as good a dose as any to start with though. Keep us posted on the effects.

Jmann, Here is the conversion for you and everyone please, check my math...(molecular weight of Asp=133.1)
1.9mmol/liter conversion to mg/50gallons
1.9mmol/L x3.8liters/gallon x50gal =361mmol/50gal
361mmoles /133.1mg/mmole =2.7mg per 50 gallons
Just find the ammount of Asp per tablet and you are in business.

 

[Roadtoad]

Robert Patterso, I just moved to Coppell, in fact I was just at the lowes in Denton. What kind of cycling do you do and where do you do it? I have been looking for some climbs with no luck!

Sir, dovrete perdonare il mio italiano fanno 10 anni che non parlo. Se l'effetto (il aumento nella crescita) è specifico a hGH, (o intero o frammento) dovremmo colaborate e clonare il ricevitore (receptor).

 

[Robert Patterso]

<a href=showthread.php?s=&postid=8797265#post8797265 target=_blank>Originally posted</a> by Roadtoad
Robert Patterso, I just moved to Coppell, in fact I was just at the lowes in Denton. What kind of cycling do you do and where do you do it? I have been looking for some climbs with no luck!

Sir, dovrete perdonare il mio italiano fanno 10 anni che non parlo. Se l'effetto (il aumento nella crescita) è specifico a hGH, (o intero o frammento) dovremmo colaborate e clonare il ricevitore (receptor).

I moved here from Ventura, CA. 2 yrs. ago. I raced mountain bikes in sport class age group racing, NORBA, and a little road racing. Mainly road for training. Six weeks after I moved here I wrecked my back and had to have surgery, that took me out for the first year I was here. This year I broke my wrist pretty bad and have been in a cast or splint for seven months, still in a splint. So no riding at all. I put in about 300 miles when I first got here and that's it.

If you ride road, sorry there are no hills to climb, this is TX. You have to go down to Austin, hill country, to find any kind of hills. If you ride mountain bike they have some pretty good single track. I hope to get back on the bike this next year. Have about 35 lbs. to loose though and in terrible shape. Been a long two years.

What do you ride? What kind of bike or bikes?

 

[Robert Patterso]

Hey roadtoad, why Texas?

 

[VWD]

WOW! What a great looking tank!

 

[ocd_mariner]

1.9mmol/liter conversion to mg/50gallons
1.9mmol/L x3.8liters/gallon x50gal =361mmol/50gal
361mmoles /133.1mg/mmole =2.7mg per 50 gallons

Solby orginally said

1.9 mmol/US gallon

Wouldn't that make it
95mmol/50gal or .7mg per 50 gallons?

I could be way off base, I just noticed that you two started from different points.

 

[solbby]

<a href=showthread.php?s=&postid=8797928#post8797928 target=_blank>Originally posted</a> by ocd_mariner
Solby orginally said



Wouldn't that make it
95mmol/50gal or .7mg per 50 gallons?

I could be way off base, I just noticed that you two started from different points.

YES you are indeed correct. I already converted L to US gallons.

0.7mg per 50gallons is correct.

 

[Sir]

<a href=showthread.php?s=&postid=8797265#post8797265 target=_blank>Originally posted</a> by Roadtoad
Robert Patterso, I just moved to Coppell, in fact I was just at the lowes in Denton. What kind of cycling do you do and where do you do it? I have been looking for some climbs with no luck!

Sir, dovrete perdonare il mio italiano fanno 10 anni che non parlo. Se l'effetto (il aumento nella crescita) è specifico a hGH, (o intero o frammento) dovremmo colaborate e clonare il ricevitore (receptor).

Intendi il recettore che si trova sui coralli??? ho fatto diverse ricerche, ma è molto difficile trovare, sopratutto su internet, articoli interessanti su questo aspetto....

diamo ormai per scontato che i frammenti del gh influenzano la crescita...
l'acido aspartico secondo me ha un ruolo molto importante...

Vi ricordo che il gh non è necessario...l'importante è garantire una ottima alimentazione ai coralli...quello è solo una spinta in più, ma bisogna essere molto cauti...la cosa migliore sarebbe quella di fare abituare il sistema al pappone e poi eventualmente aggiungere gh.

bye ! Fabio

 

[solbby]

I am dosing 1.6mg for my 120 gallon tank. I am adding it to the top off water so that it will be added slowly. The L-aparatic acid I acquired from a GNC in pure power form. As for frequency I am starting slow by adding 1.6mg every two weeks.

 

[Roadtoad]

Oops, my bad on the conversion, missed that one.

Jmann, I "finished my education" at UCSF and now I am an educator at UTSW. I am a former roadie/triathlete that dabbles in mtn biking. Thanks to my stint at UCSF and a torn rotator cuff I havent ridden much in the last couple years. Im healthy and ready to ride off that extra 20 that I seemed to have picked up.

Sir, I figured that this process is a combination of nutrition for the corals mixed with amino acids that will boost growth, that is what happens in people too. I have been interested in glutamine also, have you tried that? Grazie, molto gentile

 

[jmann]

Solbby May I inquire as to why every 2 weeks? Don't the corals ingest AA's as needed? It doesn't effect water quality does it? I can understand going slow on the cubes of food to give the reef time to build up bacteria count aqnd such but don't understand the AA's every 2 weeks. Thanks John

 

[jmann]

I just discovered a easy way of getting the cubes to release out of the the trays. I just ran the tray, bottomside up, under some hot tap water for a minute, and tapped the tray on a tupperware bowl and the cubes fell out easily. I took them all out and put them in the storage bowl in freezer. John

 

[Aqua Keepers]

<a href=showthread.php?s=&postid=8803689#post8803689 target=_blank>Originally posted</a> by jmann
I just discovered a easy way of getting the cubes to release out of the the trays. I just ran the tray, bottomside up, under some hot tap water for a minute, and tapped the tray on a tupperware bowl and the cubes fell out easily. I took them all out and put them in the storage bowl in freezer. John

How about lineing the tray with wax paper or serran wrap?