I've never been down this road, everything was great for the last 3 years since I 1st took up saltwater. I followed the advice of Aquarium services- bought a new 10 gal Quarantine tank, took an old Eheim 2215 apart and soaked everything in fresh water, put only bio mech in it, filled it and the new tank with 30% salted Ro Di and 70% water from the main tank, brought the temp to 30 deg C., same as I had previously raised the main tank to, added appropriate amounts of "Colony Marine and Ich-X", let it run for 24 hrs, acclimated my yellow Tang and Clown before putting them in and 10 hrs later (overnight) they were both dead.
The reason I'm suspecting Ich is my blood shrimps, snails, anemones and 2 marine species that look like ww1 naval mines are still doing fine in the main tank just all my fish have died.