My How To Culture Phytoplankton Guide

Posledni Czar

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What you will need:
  • Clear plastic bottles with labels removed
  • Airline tubing
  • Rigid tubing [optional]
  • Air pump
  • Light fixture
  • Fertilizer
  • Salt water
  • Phytoplankton for starting culture

Growing phyto isnt hard at all. you just need to meet its needs: Light, food, and environment. Take a 1l plastic bottle and fill it with water. I use a SG of 1.014 because i've found it works best [though I am still experimenting with this] and about 10ml of fertilizer [again the amount is up to you, whatever gets best growth]. Which fertilizer you use is also up to you, just need to make sure there isn't any significant amount of copper. I use a liquid plant fertilizer.

To prepare the bottle I make a hole in the cap, large enough to snugly fit an air line, and another smaller hole [to allow air to go out]. I run a length of airline all the way to the bottom [if you have a rigid tube that is better] and enough to go to your airpump or splitter. I fill the bottle 3/4 of the way with water and 1/8 with fertilizer and 1/8 of the phyto.

Put the cap on and turn on the air pump. For the light I use my sump light [a 23w CF energy saver screw-in type on a clip on fixture] and run it for about 16hrs or so a day. You can set up the culture anywhere as long as you have the light, I have it set up in my sump since its convenient, there is a light already and its out of the way.

The culture is ready when its reaches a good colour. You can test to see if its done by checking for phosphates and nitrates. When the tests read low your culture will no longer have enough nutrients to continue to multiply [I use the colour method myself]. At this point, I filter the culture to remove any large particles [I use a coffee filter or paper towel]. Now you are ready to use it to dose the tank or start a new culture. The Phyto should be stored in the refrigerator, and shaken periodically to prevent it from settling.


Pictures will be up shortly. I wasn't sure wether this was the correct forum, I was debating between this one and the DIY one. mods feel free to move it :)

I'd also like to put a disclaimer here, I use this method to raise phytoplankton for my tank and my brine culture and i'll use it for my copepods. I am not responsible for anything that may occur or not occur to your tank or livestock from using or not using this method.

To be updated...
 
by liquid plant fertilizer are you talking terrestrial? If so it is not formulated for microalgae and will be deficient in some respects and too much of other stuff. There is no reason not to use the proper Gulliards F/2 which is designed specifically for marine microalgae. In fact if you get the powder it tends to be cheaper and last longer then Miracle Grow or other terrestrial plant fertilizers.

https://3kserver7.com/~frank/secure...442.2113*mE6Io3&product=MICRO_MACRO_NUTRIENTS

For $20 you can get 2.2 pounds of dry that makes 10,000L of culture water.

picserve.cgi
 
This is my method, and I posted it because perhaps it'll be some help to someone. If you have critiques questions or comments post em up.

and also the Florida Aqua Farms product is Gulliards, and its my source for ferts.
 
I use gulliards personally, but you can use miracle grow or other terrestrial ferts just as well. I used them at one point.

While terrestrial fertilizers will grow some phyto's, it will grow nutritionally inferior phyto ;) Besides, as Gresh pointed out, it doesn't actually save money compared to using Guillards.
 
If it did we'd use it :lol: With over 50 million gallons of culture water processed a year any cost savings would be awesome. But alas, it's more expensive and far inferior then a proper F/2.
 
The company I work for does (Reed Mariculture), we're the worlds largest producer of marine microalgae concentrates. Currently our products are used in over 80 countries at commercial hatcheries, research facilities & universities. We also have a Marine Ornamental product line, Reef Nutrition.
 
oh cool! I've thought about getting reed products on occasion, but then I always end up deciding to culture my own. :)

I prefer culturing my own for the lab. However, I do keep a stash of Reed's concentrated phyto in the freezer as a back up to my cultures. Occasionally need it when a student doesn't keep up on the cultures when I take a few days off, or other problems crop up (usually when I'm off). Got to keep the rotifers fed :D
 
I'll take that as a no. Recycling the water would add more cost than just using new water each time, but not the most environmentaly friendly option..............
 
is the micro algae grow (gulliard's) florida aqua farm sells any good? melev's site seems to prefer miracle gro over that since melev's experience frequent crash using gulliards.

how much shipping does florida aqua farm charge? I hate their ordering system where you submit everything with credit card information and all and they don't tell you how much the shipping is going to be up front. for all I know they can charge a large amount for shipping an $8.50 Nanochloropsus culture disk. so I am thinking of using phytofeast live. I know it is good for feeding, but not sure if it is good for starter culture.

thanks
 
The Micro Algae Grow from Florida Aqua Farms (FAF) works just fine. While their shopping cart is a bit out of date in regards to calculating shipping, I've never had them charge me more than actual shipping costs.

As for the idea that Miracle Grow could be better, not a chance. If someone is having more problems with crashes while using Guillards, they are doing something wrong. Keep in mind, that particular source of info is from a hobbyist, not a professional aquaculturist ;)

With the Phytofeast live, while you can start live cultures from it, it is not a single algae species, but a mix of several. It's always better to start your cultures with a known single species so that you know for sure what you have. Those Nano discs from FAF work well for starting cultures.
 
can you give a specifc example of the shipping charge. actual shipping charge for fedex priority overnight is at least $60 coast to coast, I imagine UPS or USPS won't be too different. what is your shipping charge from florida to new york when buying from FAF?

thanks
 
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