Our tank's biology up close

I'm not entirely sure what this thing was. Amoeba like but moved quite differently and almost reminded me of a part of an aiptasia but didn't look the same as what I've seen before. Seemed to have some segmentation but maybe not.

11Months_AfterMetro_Update_ATS-0026 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0027 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0028 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0029 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0030 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0031 by Jason, on Flickr
 
Here's one of the types of amoeba's I've seen several times in the past consume organisms and split. I got to see this one consume as it went at least once but then for the first time I found this one to create a cyst.

11Months_AfterMetro_Update_ATS-0047 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0048 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0049 by Jason, on Flickr



This is right before it turned into a cyst. It sort of "reached" up and came back down forming an orb. I'm not sure but it could be because it got hit by a nematode. Videos to come.

11Months_AfterMetro_Update_ATS-0050 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0051 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0052 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0053 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0054 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0055 by Jason, on Flickr

11Months_AfterMetro_Update_ATS-0056 by Jason, on Flickr
 
Video playlist

https://www.youtube.com/playlist?list=PLN_wI2B-a8vqV8QdQFCAv7w6aLZ1V-q3a

<iframe width="560" height="315" src="https://www.youtube.com/embed/videoseries?list=PLN_wI2B-a8vqV8QdQFCAv7w6aLZ1V-q3a" frameborder="0" allowfullscreen></iframe>


https://youtu.be/Q0BYLXIlsa0?list=PLN_wI2B-a8vqV8QdQFCAv7w6aLZ1V-q3a
<iframe width="560" height="315" src="https://www.youtube.com/embed/Q0BYLXIlsa0?list=PLN_wI2B-a8vqV8QdQFCAv7w6aLZ1V-q3a" frameborder="0" allowfullscreen></iframe>


https://youtu.be/YtTaAvwyN8Q
<iframe width="560" height="315" src="https://www.youtube.com/embed/YtTaAvwyN8Q" frameborder="0" allowfullscreen></iframe>

https://youtu.be/pAfoQxXURQ4
<iframe width="560" height="315" src="https://www.youtube.com/embed/pAfoQxXURQ4" frameborder="0" allowfullscreen></iframe>

https://youtu.be/LXomBWBzyok
<iframe width="560" height="315" src="https://www.youtube.com/embed/LXomBWBzyok" frameborder="0" allowfullscreen></iframe>
 
Just after the 8 minute mark it runs into a snack in that 30 plus minute video. After the 15 minute mark it starts its transformation.
 
Last edited:
Thanks for these! I've seen those amoeba cysts in samples, but had no idea what they were.

That "segmented" or at least compartmented wormthing is really cool too.
The little dots inside almost looked like photosynthetic symbionts. Wonder if it's a tiny flatworm larvae of some sort

Sent from my SAMSUNG-SGH-I337 using Tapatalk
 
taricha said:
Thanks for these! I've seen those amoeba cysts in samples, but had no idea what they were.

That "segmented" or at least compartmented wormthing is really cool too.
The little dots inside almost looked like photosynthetic symbionts. Wonder if it's a tiny flatworm larvae of some sort

Sent from my SAMSUNG-SGH-I337 using Tapatalk
Click to expand...
Yeah, I've seen that type if amoeba before and have some cool videos of them splitting. And I've seen those cysts before too but was never sure what they were from. I was thinking dinoflagellates but this was awesome getting to see it go through the transformation and know now exactly what that cyst is. Just don't swallow them as it could lead to amoeba dysentery. [emoji4]


Or even worse Naegleria fowleri* but that's from fresh water.

The segmented "thing" I would love to find out what that is. I have not had the time to look up much on it yet. I was thinking those were food vacuoles but some kind of photosynthetic symbionts would be cool.
 
taricha said:
Thanks for these! I've seen those amoeba cysts in samples, but had no idea what they were.

That "segmented" or at least compartmented wormthing is really cool too.
The little dots inside almost looked like photosynthetic symbionts. Wonder if it's a tiny flatworm larvae of some sort

Sent from my SAMSUNG-SGH-I337 using Tapatalk
Click to expand...
Yeah, I've seen that type if amoeba before and have some cool videos of them splitting. And I've seen those cysts before too but was never sure what they were from. I was thinking dinoflagellates but this was awesome getting to see it go through the transformation and know now exactly what that cyst is. Just don't swallow them as it could lead to amoeba dysentery. [emoji4]


Or even worse Naegleria fowleri* but that's from fresh water.

The segmented "thing" I would love to find out what that is. I have not had the time to look up much on it yet. I was thinking those were food vacuoles but some kind of photosynthetic symbionts would be cool too.
 
Whoa!, something happen and this video now has over 10k views. lol

A certain recent comment caught my attention and I went to look and noticed the view count.

https://youtu.be/lNiB7dPaECI

<iframe width="560" height="315" src="https://www.youtube.com/embed/lNiB7dPaECI" frameborder="0" allowfullscreen></iframe>
 
I'm getting a new scope with quintuple nosepiece, infinity corrected plan 10x, 20x, 40x, 60x, and 100x dry objectives and camera 20MP Sony high speed 60fps bigger 1" sensor for direct full 1x C mount.

I had a very helpful and pleasant conversation with the folks over at microscopecentral.com. https://microscopecentral.com/pages/about-us

I settled on an accuscope which is a good mid-range scope that's been making them for over 90 years. microscopecentral.com is a prefered distributor for them. It's a step up from the omax and amscope. Which I found out are sister companies and re-brand a lot but are a great value scope.

The exc-350 is what I'm getting. Has a lot of accessories that can be added, 5 objectives, supports 1" camera sensor, can direct all light to just the camera top port among several other features I liked.

Can get really nice (very expensive) splan apo objectives among many others like the ones I mentioned above that are reported to be up there with Nikon quality wise.

https://microscopecentral.com/products/accu-scope-exc-350-microscope

I picked that over another labomed, accuscope, and meiji due to supporting a direct 1x C mount for 1" camera sensors.

The camera is what I started looking at in the first place and ended up with a whole new microscope. lol. My son will be happy as the omax will be his and will wrap it up for his birthday.

I've been wanting to upgrade to a higher resolution faster FPS camera since even buying the omax with the 5MP camera. I just wanted to start somewhat basic first to get a feel how involved I would be with it But I'm just loving photomicrography so much it's become a hobby on its own.

So, I was looking originally at the 18MP USB3 mounted camera sensor but started researching a bit more. I found Sony has been really advancing their sensor technology to rival that of Nikon and Canon so started there and found they make a high speed pretty advanced sensor

http://www.sony-semicon.co.jp/products_en/IS/sensor0/technology/exmor_r.html

http://www.hispeedcams.com/sony-imx183-exmorr-new-rx-series/

Which was a perfect sensor for a microscope being at 1" or under and a nice 2.4um pixel size, great for details and low light, plus the speed I'm looking for.

https://microscopecentral.com/pages/how-to-buy-a-microscope-c-mount

http://www.avcemporium.com/optical-adapters-1/

https://www.microscopyu.com/tutorials/matching-camera-to-microscope-resolution

So I went looking for this sensor. Well I found two places that had a microscope camera that included these Sony back illuminated sensors. And both were exactly the same. Looks like both Omax, Amscope, and many others rebrand from a company called Touptek.

http://www.touptek.com

Which the omax 5mp camera I have now is one and comes with their software Toupview which I really like a lot. So, I found a US distributor of Touptek cameras before being rebranded and it is cheaper that way.

http://www.avcemporium.com/touptek/

Cheaper by about $100 then the Amscope branded camera.
http://www.avcemporium.com/touptek-e3ispm-fast-usb-3-0-cmos-color-cameras-with-sony-bsi-sensors/

I'm super excited and should have both in a couple weeks.

Now I just have the phase contrast kit to get from what I originally was planning on upgrading. Geting the plan 100x dry objective with the microscope. That and I'm looking at other techniques too like DIC.

I also found how to DIY a darkfield setup for super cheap with out buying a couple hundred dollar kit. And other filter techniques.
http://www.microbehunter.com/how-to-make-microscope-filters/

RHEINBERG ILLUMINATION
http://www.intellectualventureslab.com/invent/colorful-microscopy

https://youtu.be/taKfysZ-LNY

https://youtu.be/Mkqi3fL84sg
 
That looks like a lot of fun. I'm going to have to look into getting my microscopy act back together when I'm over this cold.
 
Oh my! I got the Accu-scope EXC-350 in and I'm very impressed and very sad I don't think I'll get to play with it tonight as I have a lot of work to get done. Will see.

It's definitely a notch higher in quality and features over the Omax I had. Very solid and heavy.

Metal gears. VERY smooth, everything is. The 1x C-mount for it is focusable. Has a condenser and field diaphragm. Filter holder. Slider a d accessory slot. Tension control for the focus. Both eye pieces focusable and are large for those with glasses. 5 objectives all dry.

Man I can't wait to use it

69b451e75b984c00768d8baee0937046.jpg


b5b82d345ae5c5f5ee618e8f337721ee.jpg


6bb0b840f7d28010301f4d189c687fe1.jpg


b050cf3b4b4a24fb16afda3afab98b51.jpg


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e33288a23128d1455b95c5b824545fed.jpg


fc8a50d4adff3e0f3cb1f9208f9a5da3.jpg


bc6dc89070f69a0bfa613e64eb6b904c.jpg


ec9b9ef6710e15b27015d24c55044d30.jpg


835ba335b20e53e1106b3eff2e8bc761.jpg
 
What kind of setup will you use for pictures and videos with this setup? I've forgotten (or maybe you haven't posted) the setup you're using now.
 
bertoni said:
What kind of setup will you use for pictures and videos with this setup? I've forgotten (or maybe you haven't posted) the setup you're using now.
Click to expand...
I have a notebook that I used before with usb3 the new Touptek camera thats mounted on top with the 20MP 1" sony sensor will connect to. Still the same camera maker as the 5MP I had so still using the same toupview capture software that I do like.

It's pretty simple software to use for the basic imaging stuff but I still haven't tapped into all its potential. Like some how it can get measurements in the images, layering and stitching images, etc. I just haven't taken the time to learn those features yet.
 
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