Sorry for the delay in updating, life has been a little busy as of late. First off, the remaining fish have pulled thru nicely and are all very active and eating well.
I ran some controlled tests utilizing 2-5 gallon buckets, the test kits used were Seachem, and Salifert. I did utilize the drop method for these tests due to the water volume I was testing. The numbers you see in the photos represent drops per 5 gallons with the Seachem test. The Salifert test at this low dosage was a little tricky to read, but once the dosage was increased, it was right in line with the Seachem test. I included a picture of the Salifert test at the final concentration, which would be 16 drops per 5 gallons, 0.5mg.
Judging from the test results, a hyper concentrated bottle of Cupramine is not what I have in my possession, at least not in terms of what these test kits read.
I was really hoping the test results would yield a definitive answer as to why a 1/4 dose of Cupramine would have the toxicity to kill in a manner for which I experienced, but instead I am left with more questions, and no real answers.
I am only left to theorize that something else was amiss, but what that could have been, I have no clue. I just know that I have never had an experience like this, and have successfully run dozens of fish thru the same quarantine protocol without issue.
I am not a Chemist, nor do I know the exact chemical makeup of Cupramine, but I am certainly curious what Cupramine contains, and if anything else within its makeup could lead to this"¦