No. Think of this like a pesticide. It really does nothing to the corals or fish. The only concern is die off of zooplankton. If you had a heavy copepod population it is possible their death would cause a spike in pollution so a water change and carbon are important, but otherwise the "medicine" does no harm to anything other than exoskeleton critters: crabs, shrimp, copepods, and red bugs.
There is your answer!It's like any other medication that it gets weaker with age.
?? I did the second dose everything looks good should I still do the 3rd dose and ow long should I wait for the 3rd iv read 1 week the same as the others and iv read 2 weeks
Don't skimp out on the third dose... Do it for insurance... I sure know I wouldn't want to repeat this process ever again unless absolutely necessary. Just my .02 cents.