A while back, I created a thread called something like "Natural Filtration Methods" and I described some of the different 'natural' methods I was going to employ in order to create a healthy Microcosm for my shark breeding system, which is roughly 2000-2500 gallons. Years ago, I had read the works of Jauberts, the refuges in Monaco Aquarium, Martin A. Moe and both of his miniature Ecosystems of a Temperate zone and tropical zone through the Smithsonian. More recently, as an educated biologist, I am more interested in biodiversity including the smallest of Microflora and microfauna.
The thread never really took hold with most people but (Paul B) who is also in NY as I am, who's aquarium is over 42 years old added notes. In his threads, you can find that he adds local muds from less eutrophic, estuary type areas from even here in a temperate latitude.
I believe that the greater the biodiversity of both microbes, micro-invertebrates and crustaceans, as well as an organic carbon source can create a superior environment for marine animals to flourish. However overtime, due to competition and natural selection, the amount of diversity begins to lessen, with solitary species taking control and outcompeting the rest.
I have taken interest lately in "cryptic refugia" and have a glass tank with Live Rock pieces in it, where all gaps between the pieces of rock have been filled with sponge growth! I'm actually afraid to disturb it at this point as removing one rock would shift this natural 'mortar like sponge growth'.
Needless to say, you keep surprising me with each phase of this build!
I'm very curious of this microbe extracted from mangrove root, and what it is and what it does that attracted you to it?
Using all of these nature, locally collected shells, mud, rocks etc. can carry the risk of introducing high nutrient levels initially, and contaminants.
I'm sure you've done your homework however! The rock work has come out looking like art! Well done.