Lizz, If you need more RO water I've got the 55g drum full at the moment and you're welcome to some. I'm having a hard time with this scenario because Friday evening when I popped over the tank temperature was still normal even though it was a warm day and then Saturday morning it was ok as well. As we were talking about before, I've had some crazy (slow) temperature swings in my setups without losing stuff. If it rose up to 85 between me being there in the morning and you getting home later that day I have to ask, are you sure the heater is functioning properly? The other aspect is that the initial shrimp that went missing before you left, normally I wouldn't think that'd be enough to spike the ammonia so bad to cause these issues either but could be wrong. But with the second shrimp MIA as well... that may be it though. You mentioned other stuff is starting to look at little off now too?