<a href=showthread.php?s=&postid=6476985#post6476985 target=_blank>Originally posted</a> by Acipenser
I think we all need to get a better handle on the process of diffusion.
Definition: Movement of a fluid from an area of higher concentration to an area of lower concentration. Diffusion is a result of the kinetic properties of particles of matter. The particles will mix until they are evenly distributed.
Any dissolved compound will be evenly distributed throughout the water column. When you reduce nitrates in one area (DSB), nitrate will "flow" to that area to equalize the concentration. A sand bed is no obstacle to diffusion. A semi-permeable membrane - yes, sand - no.
The point is that the organic matter does not have to get into the bed to be processed. The organic matter can be broken down anywhere by any creature. It will be converted to ammonia. Ammonia can be processed anywhere two species of bacteria can colonize (glass, pipes, live rock, sand, etc...) and oxygen is present (alkalinity must be present too, but that is another story). The nitrate will get to the lower areas of the DSB (bucket or in tank) no matter what!
I also feel that diffusion is sufficiently fast to work for denitrification in a DSB.