Prodibio

<a href=showthread.php?s=&postid=7842557#post7842557 target=_blank>Originally posted</a> by TryTheChi
... Just to check my understanding - zeolites may have an advantage over other media by being an kind of hospitable petri dish, with some impact on the makeup of the mulm? ...
I can't address what advantage(s) "zeolites" may or may not have ... regarding Prodibio ... without specific information about either the specific zeolite being applied and/or the bacterial strains involved. That being said ...

All I can comment on is my experience with the ZEOvit media and ZEObak pairing, and the "Sebralla Report". Given the way that nutrients (not just PO4) were rapidly reduced in my system (ZEOreactor in a 60G with <7lbs. LR), the rapid growth of bacterial biomass when the carbon source was overdosed, and the numbers from the Sebralla report with regards to Fe and S ... yes ... I believe (hardly empirical) ... that Korallenzucht's zeolites provide a productive substrate for the strains in ZEObak. Whether any zeolite will provide a productive substrate for any bacterial strain seems to me to be an open question (and I suspect that the answer is no).

BTW ... Sulfur from 348 to 234 ... Fe from 12.3 to 7.9 ... consider what the limiting factors for sulfur-reducing and iron-oxidizing bacteria (either inoculated or extant) might be in our aquaria, and consider whether or not it's the ammonia ... or the carbon ... which is also limiting.

JMO ... ;)

I am aware of no literature reference which indicates that the substrate influences the composition of macro-aggregates directly, unless the particulates around which the macro-aggregates form are derived from the substrate itself.


JMO
:D
 
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<a href=showthread.php?s=&postid=7842557#post7842557 target=_blank>Originally posted</a> by TryTheChi
Is the latter a feature of the chemical makeup of the substrate or the environmental conditions ...
Macro-aggregates have been documented to form around orgainic particulates, inorganic particulates, and air bubbles. I am aware of no empirical data documenting how macro-aggregates are formed in aquaria, except with regards to air bubbles (Spotte, 1979).

I speculate that macro-aggregates in aquaria primarily form around organic particulates ... hopefully solbby's experiments can shed some light on this. If ... and that's a big speculative 'if' ... I'm correct, then "environmental conditions" might well drive macro-aggregate production, and the presence of some mechanical apparatus which introduces organic particulates into the water column might be significant.


JMO
:D
 
<a href=showthread.php?s=&postid=7842557#post7842557 target=_blank>Originally posted</a> by TryTheChi
... Thinking about we might expect expect to observe in home captive reefs, if we were looking to verify the hypothesis - we could observe through tests that NO3/PO4 were reduced faster? ...
What hypothesis? ... seriously.

Personally, I'm still a few kilometers away from anything resembling a workable hypothesis regarding exactly how bacterioplankton filtration configurations do what they do. Despite some intriguing correlations between what I'm seeing, measuring, and the academic literature ... I'm hard pressed to describe my observations and suspicions as anything more significant than "well-researched storytime".

But I'm working on it ... :D

Empirical data regarding water column chemistry and bacteria export via foam fractionation (both in terms of quantity and composition) might prove useful in helping to better understand how products like Prodibio do what they do.


<a href=showthread.php?s=&postid=7842557#post7842557 target=_blank>Originally posted</a> by TryTheChi
... What other observational indicators may there be? - water clarity? ...
An interesting candidate ... certainly ... and we have relatively inexpensive tools to measure and partially quantify "water clarity" in terms of turbidity (and literature documenting optical differences between oligotrophic vs. eutrophic water columns). But in the absence of a working hypothesis (which accounts for other "interfering" variables) ... how would we usefully apply such observations?


JMO
:D
 
<a href=showthread.php?s=&postid=7842557#post7842557 target=_blank>Originally posted</a> by TryTheChi
... Greater coral nutritional value of the bac biomas as seen through growth rates or coloration? ...
This is my suspicion ... definitely ... and the potential for bacterial biomass to contribute to the energy budget of corals is well documented in the academic research. But exactly how, and to what extent, products such as Prodibio generate nutritional enrichment of corals remain open questions (not to mention questions regarding even a rudimentary quantification of what constitutes changes in either growth rates or "coloration") ... yes?


JMO
:D
 
<a href=showthread.php?s=&postid=7842557#post7842557 target=_blank>Originally posted</a> by TryTheChi
... If that is the case, then our feeding strategies for the bac strains become THE focus after ensuring we have the right balance of strains? ...
I have no idea what "the right balance of strains" means. I have not seen ANY empirical data (N=20; p=0.05 .... Mr. Borneman and Dr. Holmes-Farley cannot be more right about the need for this kind of standard) regarding the composition of the bacterial biomass which exists in our captive marine microcosms.

I'm waiting for solbby's data on many levels ... :D



Long have I ranted ... it's the biofilm. It seems to me that the fundamental difference between BB Berlin system water columns, SSB Berlin system water columns, and DSB system water columns in comparison with the water columns generated by Prodibio, ZEOvit, Polyplabs, Ultra-Bio, or vodka dosing ... is the difference between water columns which are both carbon and nitrogen limited, and water columns which are nitrogen limited, but not carbon limited.

What carbon source to feed which bacterial strain may prove to be critical ... and a baseline distinction in evaluating various product lines. It may also turn out to be the key for integrating various product lines into so-called "hybrid systems."

Once we figure out how this difference effects coral energy budgets ... all the other issues will fall largely into place, our ability to evaluate the various product lines will improve, and we will have stumbled upon another powerful husbandry option in terms of manipulating coral growth & coloration.

But until we figure out this fundamental dynamic ... we're just telling each other stories, and sharing pictures of the anthozoan kids ...

... ;)



JMO ... HTH
:D
 
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It all sounds interesting, but is so expensive! While I'm not opposed to spending money on my reef, I'm not yet convinced, but will be watching with interest.

I don't understand a few things, like what exactly distinguishes this bacteria from any of the others we naturally culture in our systems, and what makes the zeolite a better media than live rock, sand ect...? Also I gather that this is an aerobic bacteria, how does it eventually lower nitrates to undetectable levels if so? Is this more a function of the zeolite than anything, by that I mean could you make a reactor, obtain some zeolite, feed it an organic carbon source and culture bacteria already present in your system? Has anyone tried to DIY this concept?

Guess I need to do more reading.
 
<a href=showthread.php?s=&postid=7848051#post7848051 target=_blank>Originally posted</a> by reefshadow
It all sounds interesting, but is so expensive! While I'm not opposed to spending money on my reef, I'm not yet convinced, but will be watching with interest.

I don't understand a few things, like what exactly distinguishes this bacteria from any of the others we naturally culture in our systems, and what makes the zeolite a better media than live rock, sand ect...? Also I gather that this is an aerobic bacteria, how does it eventually lower nitrates to undetectable levels if so? Is this more a function of the zeolite than anything, by that I mean could you make a reactor, obtain some zeolite, feed it an organic carbon source and culture bacteria already present in your system? Has anyone tried to DIY this concept?

Guess I need to do more reading.

Hi Reefshadow

The manufacturers of the various probiotic products are developing the idea that bacteria strains all naturally occur, but in a closed environment of reef tanks the range of bacs become limited due to environmental conditions and lack of appropriate elements.

All probiotic methods aim to regularly re innoculate the captive reef with a range of bac strains and provide these with appropriate carbon sources to get the bacs to florish.

The outcome one can expect to see is a very low nutrient picture, resulting in the decline of nurtient dependant algae including symbiotic algae., the latter allowing for the underlying color of an sps to be more visible.

If one has undetectable nutrients and great coloration anyway, I cant see there would be any benefit in adding bac strains and carbon sources.

In the zeovit method, the zeolites have two functions: ammonia adsorbtion (although this is disputed by some) and as a biological filter. In the zeo method the zeolites are shaken daily to prevent the zeolites from clogging up with bacs ( and therefor enable adsorbtion - if it does) and to release bacs into the water colomn which then act as food to the corals promoting growth and vitality, and therefor supporting coloration. The residual bacs in the water colume are exported by protean skimmers. The bigger the better.

In this model, the bacs in the water colume are needed as sps, lps and other corals have less energy available to them through the symbiotic algae.

In all bac innoculation systems - the bac solution must have denitrifying strains, and we must have appropriate anerobic environment for these to function.

Some think that zeolites do not provide this environment as thier structure is too dense. ZEOheads recommend 3 hour on and off phases for the reactors to provide this if nutients are high.

For prodi and zeo and others- the denitrifying bacs will live in the LR and/or dsb.

I cant see any reason why one cant DIY the concept to get the benefits. Vodka is a DIY carbon sourse, but its critics say that it is an indescriminate source of carbon, with potential negative effects. Having said that people get cynobacter with prodi and zeo too if carbon and aminos are overdosed.

This is the strategy I'd use to DIY a probiotic system based on my experience of using both prodibio and now the full zeo system:

1) Select the cheapest bac strains available, and the cheapest carbon source designed to support these. You could even leave the innoculation and see what happens.

IME Prodi digest has produced the greater impact on clarity in the water column compared with zeo bac. Maybe polyip labs are good too? This can also be stored for longer than zeo bac.

2) Feeding the bacs is important, and ZeoFood7 allows for much greater flexability of dosing than prodi TPIM. I dont know if the two differ significantly but having used both my NO3 and PO4 remain 0. Both contain carbon, amino acids and vitamins.

3) Construct a DIY reactor which you can shake daily. The zeoforum has some examples for $20.

4) Break up some live rock so that there is a mix of sizes from 3-4 mm to 60 - 70 mm. The larger sizes will host anerobic bacs. LR should already have a range of bacs in there if you want to miss out the innoculation element. Siporax or sintered glass medium could be good too, but would definately benefit from innoculation.

Dose Prodidigest into the reactor every 2 weeks, and feed with zeofood 7 as per the zeoguide. Shake the LR in the reactor daily to provide the mulm for the corals.

I cant see why this system would not capture most/all the benefits of the other systems, and would be cheap to get going.

The main possible limitations maybe that if zeolites do infact adsorb NH3 in seawater, there may be a difference there, but I'v not been able to find any answers as to the benefits of this compared with letting the bacs do all the work. The other factor is that the LR needs to be very low in PO4 ie fresh.

All of the above may take you to very low/ undetectable NO3 & PO4, which sets the foundation for color enhancement. The addition of amino acids will be important to prevent lightening/ bleaching, and the addition of major and minor elements throuh w/c or other means may further promote coral health and pigmentation.

Just some thoughts. Will be very interested in your experience if you go for it. Pls do the before /after pic thing.:D

Cheers

Simon
 
<a href=showthread.php?s=&postid=7848213#post7848213 target=_blank>Originally posted</a> by TryTheChi
... to prevent the zeolites from clogging up with bacs ...
The white particles are dyed latex beads passing through the channels of a biofilm ...

xaqua_GoWithTheFlow.gif


Extracted from:
Biofilms: The Hypertextbook
Section 4: Do Water Channels in Biofilms Eliminate Diffusion Limitation?

Please ... may we forever dispense with the silliness that biofilms form some kind of mysterious, impenetrable barrier through which compounds dissolved in solution cannot pass?




JMO ... :D
 
Thanks Simon

Thanks Simon

Simon,

I really appreciate that last post. As a novice it helped me understand much of what has been 'in the shadows'. Good job guy.

Andy
 
<a href=showthread.php?s=&postid=7848508#post7848508 target=_blank>Originally posted</a> by mesocosm


Please ... may we forever dispense with the silliness that biofilms form some kind of mysterious, impenetrable barrier through which compounds dissolved in solution cannot pass?

JMO ... :D

Lol Gary - reminds me of a monty python sketch ... 'too silly - far too silly'. Nice pic btw - thanks - keeps things robust;)

Below is a quote from zeoguide v1.02:

"Rinse the ZEOvit material ...............
It is important to clean the material daily to maintain optimum performance. The filter specifically designed for the system makes it possible to accomplish this task within a minute."

Its refering to the benefit of cleaning the material - it later references the benefit to the corals of the mulm - two seprate outcomes from the same process. What d'you think?

Pleasure Andy :)

Cheers

Simon
 
Thanks very much for the reply, Simon. It does help to demystify it somewhat. A few more question/thoughts...

but in a closed environment of reef tanks the range of bacs become limited due to environmental conditions and lack of appropriate elements.

So is a range really that important, and why if so? Do we see different advantages to different strains, as they effect coral health and water parameters? -Beside the aerobic/anaerobic bacteria functions as they effect water params-. So the corals are feeding off the bacterial 'mulm'? What is the advantage of the system as opposed to regularily blasting mulm and detritus out of the live rock, or vaccuming the sand. I notice that my corals really respond well when I do this.

Are the bac strains employed in this method 'special' and not usually found in aquaria, or is the carbon source just their limiting factor and so we don't have them proliferating in a 'normal' setting?

Don't we naturally end up with a only few strains anyway, those that are able to best take advantage of the various conditons that favor them? So is reinnoculating really of benefit, if conditions continue to favor those bacteria? I guess I mean that if I was to start adding a carbon source, like vodka, sugar or similar, bacteria would proliferate to take advantage of the food source. Why buy it in a bottle, does it really need to be be artificially introduced, and reinnoculated periodically? Ok, hmmm... maybe I don't know what i'm asking, lol. This is all fairly new to me, but I think i'm beginning to understand the concept.

the zeolites have two functions: ammonia adsorbtion (although this is disputed by some) and as a biological filter.

Is this zeolite the same media that is commonly sold for freshwater tanks? Safe to use in a DIY concept?

Vodka is a DIY carbon sourse, but its critics say that it is an indescriminate source of carbon, with potential negative effects.

So the food used to fuel the bacteria would seem to be of some importance, what makes the food used in these systems 'special'? I was kind of wondering if 'food is food is food', perhaps those that have experienced problems are just adding the carbon source faster than the bacteria is proliferating, in effect 'overdosing'? What about sugar, is that less complex than vodka? Easier to take advantage of if you're a bacteria?

Anyhoo, just some ramblings...;)
 
reefshadow
one of the manufacturers claims 7 or 9 different strains of bacteria. either polyplab or prodibio. must be prodibio because they claim what you add is most efficient for approximately 2 weeks. therefor redosing is needed every 2 weeks.
I figure mulm can be obtained from cleaning your glass and blowing off your rocks and things of the sort. from what I have read the zeo rocks absorb some ammonia before it is broken down then the bacteria go to work on it right there. I don't know if any of it is true it's just what I have read along the way.

the sugar differences have me clueless. I do know the polyplab carbon source says enriched with amino acids. so I guess that makes it better than vodka or plain sugar. but does vodka have amino acids in it?
 
one of the manufacturers claims 7 or 9 different strains of bacteria. either polyplab or prodibio. must be prodibio because they claim what you add is most efficient for approximately 2 weeks. therefor redosing is needed every 2 weeks.

I guess I just really don't understand why redosing is necessary? From my limited understanding of bacteria, they will continue to proliferate if conditions are met. I don't understand why, if the media and food is appropriate, they would need to be reintroduced periodically. Also, if you start out with 7 or 9 strains -or whatever- of bacteria, and conditions favor only a few strains long term, why keep introducing a culture that is not 'viable'? Do you get what I mean?

I dunno. I guess this is all speculation for me, but something here just does not make sense from my laymans understanding.

the sugar differences have me clueless. I do know the polyplab carbon source says enriched with amino acids. so I guess that makes it better than vodka or plain sugar. but does vodka have amino acids in it?

Hmmm, well I would think so, if it has any protein in it. Aspartame does, lol. -NOT suggesting aspartame-
 
This is my understanding...if any of this is incorrect feel free to speak up.

Our tanks cannot maintain the environment for the specialized bacteria. Our tanks are better suited for less effective forms which out compete these "special" bacteria. Cyano is an example. By dosing these special food sources and the bacteria themselves we are trying to maintain them and allowing them to compete with other bacs. The bacs also get skimmed out...(Meso is that article on skimmate out yet?)

We put such effort in keeping these specialized bacs because they're special :) They reduce nutrients and feed the corals in ways the easier to keep bacs cant.

The freshwater zeolites are not the same as the zeovit ones...I would not suggest using those...there are various types and blends of zeolithes out there.

As far as I know vodka doesnt have any amino's in it. Or at least ones that would benefit our systems...There are certain aminos that are utilized by bacs and corals... If you want specifically which amino's have been proven, i suggest looking at meso's articles.

On Vodka being an indiscriminate food source...these could be. I'm not sure if I buy it... I would think the additional things needed for the special bacs would be the things like aminos and Fe...maybe meso can chime in on this?

Our $.02

eric&flint
 
<a href=showthread.php?s=&postid=7853404#post7853404 target=_blank>Originally posted</a> by Flint&Eric
This is my understanding...if any of this is incorrect feel free to speak up.

Our tanks cannot maintain the environment for the specialized bacteria. Our tanks are better suited for less effective forms which out compete these "special" bacteria. Cyano is an example. By dosing these special food sources and the bacteria themselves we are trying to maintain them and allowing them to compete with other bacs. The bacs also get skimmed out...(Meso is that article on skimmate out yet?)

We put such effort in keeping these specialized bacs because they're special :) They reduce nutrients and feed the corals in ways the easier to keep bacs cant.

The freshwater zeolites are not the same as the zeovit ones...I would not suggest using those...there are various types and blends of zeolithes out there.

As far as I know vodka doesnt have any amino's in it. Or at least ones that would benefit our systems...There are certain aminos that are utilized by bacs and corals... If you want specifically which amino's have been proven, i suggest looking at meso's articles.

On Vodka being an indiscriminate food source...these could be. I'm not sure if I buy it... I would think the additional things needed for the special bacs would be the things like aminos and Fe...maybe meso can chime in on this?

Our $.02

eric&flint

Hi E&F

Not quite.......:)

1) the bacs that are dosed are naturally ocuring, but the maturation precess of a reef tank doesnt always allow the range to thrive. Cyno can be a sign of OD on aminos

2) The bacs arnt special - they havent been genetically modified. Zeo bacs are not known - prodi bacs are on the packet. There is no robust info in the public domain that I have seen on the bac cultures.

3) ZEO are very clear only to use thiers to avoid trouble with the water. However Tunze and others with a good rep also produce them. Zeolites are at best a useful tool thats all. They are not a key component of a probiotic approach, although the zeo ones are to the zeo method.

Just curious - why do you have 2 names?

Cheers

Simon
 
Simon-

What i was trying to say is that our tanks do not create a level playing field for the desired bacs. In the ocean this is a different story and is one of the amazing things about plankton and the millions of bacteria out there...they all have competitors that do simmilar or exact things yet they still co-exist. Prodibio and zeo allow us to have more of these bacteria co-exist peacfully.

Special Bacs is just the name I gave them to distinguish them from other bacs...As for zeo saying only use their bacs, well thats just what zeo says about everything...who knows though it could be true.

As for the zeolites...we really dont know anything about them and their effect on bacteria cultures...it's another zeovit mystery.

And to answer your last question there are two names, because there are two people...Eric and Flint :)

Probiotic ? :lol: we really need to come up with a different name.

eric&flint
 
Greetings from the other side of Atlantic! :D

A little late but here I am to try the probiotic approach to reef keeping.

But after a few emails changed with the people from Probidio I have a few doubts witch I would like to clarify if possible.

Biodigest

The dosage size. I've got a 500 lt aquarium. The answer from Probidio regarding this it that I should dose the entire vial in the aquarium, since they recommend 1 vial to aquariums up to 1000 lt.

I've read a few posts here that try to relate the over dosage of Biodigest with some RTN cases.

So I’m a little confused about this subject. Any recommendations?

Activated Carbon

Most of the people run's carbon in a 24/7 basis as I do it actually without the probiotic products. From Prodibio I’ve got the following answer

"Change your carbon the week when you don't add BIOPTIM"

I change my carbon once a month. Do you guys think that the Probidio answer should be considered?

Storage

Where do you guys store your Probidio products?

Natural Sea Water

Does anyone use the probiotic approach with NSW? I didn't have in the past (the aquarium is 1 year and 3 months old) any case of hairy algae, cyano, diatom or other types.

Reefbooster and other products schedule

Dosing Reefbooster. From Probidio I’ve got the following

"After the water change wait one day or two before adding the products. I think it would be better to add REEF BOOSTER once and wait 48 h after to add the Bio digest and BIOPTIM "



Is anyone doing this? Or are you dosing all the products in the same day.

Can you help me making a schedule for the dosing of the products taking in consideration that I perform a 15 % water change on a week basis.

Days

1 - Water Change
2 -
3 - Biodigest / Bioptim
4
5
6 - ReefBooster
7
8- Water Change

Don't you think that leaving Reefbooster only 2 days in the system without a water change is a little waste? Should I start to do my water changes only 2 times / month or should I add the Reefbooster on the same day that BioDigest / Bioptim?

System Addiction

Does anyone have any idea about the degree of the addiction of an aquarium in this probiotic approach? I don't believe that the bacteria population start to decrease immediately, but in a mid-term timeline something should happen...

Finished my questions :)

I'll try to keep a log about this approach and try to make some photos to fulfill it.

BTW here are some photos of my aquarium (thanks to João for the beautyfull pics) 80 % made of frags, since we are a very small market and with don't get even the half of the variety of the USA and German market
:mad:

DC00.jpg


DC0.jpg


DC01.jpg


DC1.jpg


DC2.jpg


Cheers!!
 
re: sugar vs vodka dosing... Do a search on sugar dosing and you will find a long list of disasters. Although they are supposed to be an equal carbon source, for some reason people seem to have horrible results with plain sugar. There seems to be no commonly accepted idea why vodka seems to work. I for one know that it does. It seems that many that got into trouble with vodka dosing (without using prodibio) seemed to dose too much vodka without doing any research. I vodka dose on my nano (11 drops for 25 gallons) but on my 160 system I'm sticking to digest and bioptim. After 6 weeks, I have not yet seen a significant change in nitrates and phosphates. The system has a fairly heavy bioload and it is a relatively new system so I'll probably stick with prodibio for about four months. If I don't see the desired results I will switch back to vodka dosing to see what it does. I really hope it works as I would much prefer to dose every other week rather than daily.
 
Duarte Conceicao, your tank looks really nice to me. I really can't answer a lot on this topic because I personally don't have a lot of experience nor comprehension. I do know that I was overdosing BioDigest badly (3 vials in a 280g tank), and each time I'd lose one coral (fragment fortunately) within 48 hours.

Your photographer did a great job.
 
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