Vibrio

[Kathy55g]

Anyone here have experience with Vibrio infection killing off juvenile clownfish?

I first heard of this bug at IMAC 2005, presented as a major death knell for seahorses. Now I've learned that it has infected a friend's growout system and is killing a percentage of new transfers to that system.

How does one get rid of Vibrio?

 

[ediaz]

There are a few vivrio bacteria but as reference

The first step is to keep the system super clean.

It is treated with oxytetracycline or sulfadimethoxine/ormetoprin mix in the food.

Ed

 

[olin]

Vibrio is actually my specialty- do you have a specific species that you are concerned about? I ask because there are hundreds of species according to some classifications, and pathogenicity can range from harmless to fatal even within a single species, depending on the acquisition of virulence genes. They are ubiquitous in marine water. There is a near 100% chance that any given marine aquarium will have Vibrios present, and elimination of them as a whole is an excersise in futility, but within a given tank it can be dealt with, especially in larval rearing. They are great decomposers and are one of the primary bacterial species responsible for consumption of uneaten food and excrements in most aquariums. That said, there are certainly species that are highly pathogenic and quite fatal in species ranging from humans, to fishes, and corals. If you can provide some specifics as to symptoms, tank conditions, etc, I might be able to point you in the right direction as far as control is concerned.

 

[spawner]

Kathy, would you like some vibrio specific plates (TCBS) to test your system or any sick fish. This way you could at least know it was vibrio or not.

 

[Kathy55g]

It has been identified by a lab my friend sent the dead fish to.
It is the strain V. parahaemolyticus. Any info you can give would be very much welcome.

My latest thought is that we might be able to control it with pH. Clownfish can apparantly tolerate down to pH 5, and there is a publication that states that vibrio will not grow at acidic pHs.

Come to think, people use lime juice to "cook" oysters and squid for raw consumption, don't they. Perhaps pH plays a role there.

 

[ediaz]

What a small world Andy, the diagnogsis was made by Snekvik at WADDLA I beleive.

Ed

 

[spawner]

 

[Luis A M]

<a href=showthread.php?s=&postid=7669760#post7669760 target=_blank>Originally posted</a> by olin
Vibrio is actually my specialty- do you have a specific species that you are concerned about? I ask because there are hundreds of species according to some classifications, and pathogenicity can range from harmless to fatal even within a single species, depending on the acquisition of virulence genes. They are ubiquitous in marine water. There is a near 100% chance that any given marine aquarium will have Vibrios present, and elimination of them as a whole is an excersise in futility, but within a given tank it can be dealt with, especially in larval rearing. They are great decomposers and are one of the primary bacterial species responsible for consumption of uneaten food and excrements in most aquariums. That said, there are certainly species that are highly pathogenic and quite fatal in species ranging from humans, to fishes, and corals. If you can provide some specifics as to symptoms, tank conditions, etc, I might be able to point you in the right direction as far as control is concerned.

Olin,great to have a Vibrio expert chime in I think that Vibrio pathogens might be the cause of most marine larval mortality.
Which of the following methods would you address for a Vibrio-free larval or experimental system?

UV
Ozone
Antibiotics
Chlorination/dechlorination
Probiotics
Tetraselmis "green water"
None of the above but keeping pathogens away in a strictly oligotrophic system

 

[Kathy55g]

back from vacation

back from vacation

<a href=showthread.php?s=&postid=7669760#post7669760 target=_blank>Originally posted</a> by olin
Vibrio is actually my specialty- do you have a specific species that you are concerned about? I ask because there are hundreds of species according to some classifications, and pathogenicity can range from harmless to fatal even within a single species, depending on the acquisition of virulence genes. They are ubiquitous in marine water. There is a near 100% chance that any given marine aquarium will have Vibrios present, and elimination of them as a whole is an excersise in futility, but within a given tank it can be dealt with, especially in larval rearing. They are great decomposers and are one of the primary bacterial species responsible for consumption of uneaten food and excrements in most aquariums. That said, there are certainly species that are highly pathogenic and quite fatal in species ranging from humans, to fishes, and corals. If you can provide some specifics as to symptoms, tank conditions, etc, I might be able to point you in the right direction as far as control is concerned.

Mortalities are highest in a large clownfish grow out system where probably 2000+ clownfish survive and grow.
It is new juvenile transfers that get sick and some or all die.
Ozone is used in a protein skimmer, no charcoal
Large biofiltration and mechanical filtration changed daily
Siphon detritus daily
tanks wiped down weekly
17% water change weekly using carbon and DI water, Instant Ocean salt
Sp. Gravity 1.009
pH 7.9

Any ideas?

 

[spawner]

Juv. Nutrition. You guys are really cheating the system with all this rotifers and artemia; has to bite you some where. But maybe Doug will give you the real answer. JL has been fighting this as well.

 

[Luis A M]

Caterina,come e stata lÍtalia?
Are your data from some particular hatchery?
Waiting for Olin to join...

 

[Kathy55g]

<a href=showthread.php?s=&postid=7695175#post7695175 target=_blank>Originally posted</a> by Luis A M
Caterina,come e stata lÍtalia?
Are your data from some particular hatchery?
Waiting for Olin to join...

Hola Luis,
Went to Wisconsin this time. Still don't know any Italien.

Data are from JL's place. We have been conversing by email.
K

 

[spawner]

Tank data or vibrio.

 

[Kathy55g]

both. Thanks,
K

 

[olin]

Hi all, I just got back from a week in Mexico and have to get some water samples into analysis-so I will write back this evening. Didn't want anyone to think I had disappeared.

 

[Kathy55g]

glad you are back! We await your word....

I have to be gone for a couple of days, so I hope it comes tonight!

 

[Kathy55g]

Olin, my friend, where are you?

 

[Kathy55g]

bumparoonie.