The first objective when dosing organic C( carbon) is to proliferate bacteria in the aquarium that will take up inorganic nitrogen(N) and inorganic phosphate(Pi) commonly referred to as NO3/ nitrate and PO4/orthophosphate).
Hi Tom,
a question for You that arise from a recent experience.
I'm cycling a new tank, 1200 liter net volume, which actually contains only LR, pumps and skimmer. There is no light yet and no fish, coral or other invertebrate.
When I put LR in the tank, after they cycled for 3 weeks in a separate tank with a skimmer, the water had NO3 0,5ppm and PO4 0,1ppm
I added a reactor with NBP and on day 2 I had a bacterial bloom. I couldn't understand why so low values of inorganics (at least NO3) could allow a so quick and large bacterial proliferation. BTW I reduced NBP to 1 cup (about 100ml). After 2 days water came back nearly, but not totally, clear.
Slowly NO3 lowered to 0,1ppm, PO4 remained 0,1ppm.
Thinking there was a nitrogen limitation that didn't allowed for further consumption of phosphate, I added 2,5ppm nitrate, using NaNO3.
I measured NO3 on alternate days for 2 weeks and NO3 remained invariably 2,5ppm, without any reduction.
I've always thought that heterotrophic bacteria use inorganics NO3 and PO4 to grow, so I opened this topic to get some help:
http://reefcentral.com/forums/showthread.php?t=2443286
It seems to come out that this bacteria don't use inorganics. I can't face this fact I strongly believed for years.
Effectively facts seem to support this thesis:
I had a bacterial bloom while nitrate were already low (I guess LR leak lot of organic matter that fueled bacteria).
Bacteria continued to grow (demonstrated by the slightly cloudy water) but NO3 and PO4 didn't decrease.
Some days ago I removed NBP. After 3 days water has come back perfectly clear, and skimmer can't remove anything from water now, demonstrating that bacteria were actively proliferating, without the use of NO3 and PO4.
I think the absence of light makes this trial even more interesting and better understood, because avoid the confounding factor light could have been.
What do you think about?
Luca