I read all 8 pages and if I'm not mistaken......
Ormet used about 5g of Prohibit to 500 ml of RO water to create the potion. Then the potion is added at 2 ml per gallon of tank water.
However, I missed how long was the treatment before water change?
Now, in the meantime, I tried it myself.
I got a precision scale, cross checked with a calibration weight to make sure it is exactly on point. Then I add exact 5.1 gram of Prohibit to 500 ml of RO water.
To test, I captured several AEFW and placed them into container with 4 cups of tank water. From the above noted concentration, I would be adding 0.5 ml of potion. I observed the AEFW's reaction every 5 minutes. After 15 minutes, the AEFW looked unaffected at all...cruising the container wall. So, after that, I add additional 0.5 ml potion every 5 minutes to try to get a reaction from the AEFW. Finally, the AEFW look shriveled at 4 ml. So that would be 16 ml to every gallon of tank water.
What did I calculate incorrectly to deviate so much from the 2ml / gallon?