The WATER is a fairly minor locale for the bacteria that will keep your tank proper. The place the bacteria really live is the sand and live rock, and not just the surface of either of these, but deep inside. They get there by eating and dividing, eating and dividing---ie, growing big enough for two and then becoming two. And getting the tank fully cycled means having far, far, far more bacteria than comes in that bottle. TIME and good conditions for the bacteria are the only thing that can get it there. You can imagine it takes a while for bacteria to populate a rock. I know you're in haste to have it all perfect, but this is a process that doesn't work any more than you can toss seed out onto bare dirt and plan a garden party for next Friday. READ the sticky notes atop this forum. There's a lot more to this than barely cycling.