progress!
progress!
I started my phyto cultures Sunday afternoon. I'm using 750ml glass bottles (old wine bottles). Acid washed the vessels, bleach sterilized my media for 24 hours, and then de-chlorinated each vessel before inoculation. Using separate air pumps per culture, because I cannot find .2 micron air line filters. Using 2 part f/2 product from fritzpet.
Currently running 1 culture bottle of Nanno and 1 bottle of Tetra. So far so good. I think I noticed them slightly darker yesterday than the day before. Using 'Phyto2' cultures from aqua-tech co. I'd already bought thier product from a LFS in Schaumberg just by chance (a week+ ago), and then I met the owner at a frag swap Saturday. Seems like a super great guy that offered to help in anyway he could through email. I didn't even realize I had bought his product until after I was home the next day getting ready to inoc my cultures. He is confident that thier product is superior if you want pure and live phyto cultures. I saw a video where he was at a show with a microscope challenging anyone to bring thier phyto product to compare.
I will be bringing samples of my first cultures vs the originals to work some day to compare under the microscope, and post my progress.
!!If anyone has any resources or references (preferrably with pics) I'd LOVE to have them, to know what I'm looking for under the microscope!!
In addition to all the phyto stuff, I noticed that I think my momma clown is carrying eggs again!! So, I'll post again when I see some eggs lain. So they only missed or were delayed by about a cycle from all the problems while I was gone. I should be able to be ready to gather this next batch, but I don't know if I will or not (being the first after all these upsets). We'll see if I have the willpower to not gather them!...
I also found a local source for rotifers, in a town not too far from me. He also raises clowns. So i'm looking forward to meeting him and picking his brain, as well as getting some rot cultures going!!
Anyone know how much volume per week of live phyto that a 4 gal rot culture might consume? I don't want to get a culture started without knowing I have enough to feed them. (I have read yeast and even v8 juice will do the trick) So I'm guessing that I could sustain a rot culture with either of those, and switch to phyto a few days before I need them for the fry. But, again, I have no reference on how much of any of these to feed. I may have to read up more on these methods...