<a href=showthread.php?s=&postid=7337672#post7337672 target=_blank>Originally posted</a> by ZoeReef
Simon..
Simon..(or anyone) did I miss how the Iwan method could might be introduced in a startup tank.....We're setting up a 180g system (120g +20g +20g +sump) at the end of May.
Being fairly new I would really appreciate your thoughts on where you might introduce this method on our new startup. I will have about 200lbs LR and a 5" fine aragonite bed.
Hi Zoereef - so you read it from start to finish!! Phew

Iwan really is an inspiration
I just chip in with summaries to help me understand and hope Iwan will correct these if I get it a weebit wrong.
Yes you can start off, and in addition to robs advice would sugest the following:
Get as close as you can to calculating net system volume as later on some of the zeo elements need a bit of precision in dosing levels. Forgive me if you know this, but to calculate net volume from new Calculate all the inside glass measurements etc to get gross. Construct LR structure and measure how many gallons are displaced, then do the same with the DSB.
The first phase is innoculating a fresh tank with bac strains with Bio digest, and as rob said they recommend 1 amp of biodigest per 60 ltrs or 15 usg - you would need approximately 9 vials for the first innoculation if your net volume is 570 ltrs or 140 usg, and add 2 or 3 vials of TPIM - you are probably closer to 3 than 2 according to the instructions. From week 2 you'l need 1 digest and 3 TPIM each fortnight.
As rob says you may wish to leave off with the booster until you have a polip bioload of any significance. On the otherhad, if the LR is full of actual life then booster may be helpful in sustaining it. When you start dosing booster you'l need 1.5 vials each fortnight.
You can also choose whether or not to run zeostart2 once you start feeding the fish. Its not very expensive to use so its up to what you notice. If you see some undesirable algae then daily dosing of this can help. Start 2 contains more than just carbon for bacteria apparently - but we dont know what.
During this start up phase it may be worth considering a PO4 media to take up and phosphates leaching from the LR and dsb. Floridot has had professional tests done that indicate that the bacs have limited value in dealing with inorganic phosphates.
The second phase will be when you start adding sps. By this time (lets say 4 weeks after start up if you ca/mg/dkh/ph are all in line and stable) the tank will still be immature biochemically, but much more able to sustain hardier sps than non innoculated tanks at this point in the time line, you will have an nutrient poor environment. The caveat is the extent of any die off from the LR. If PO4 and NO3 are 0, start stocking with hardy sps and watch them adapt. Dependant on the species and its history you may find some brown out for a while and others lighten up to the edge of bleaching. For those that are heading down the bleaching road, begin the slowly adding AAHC and CV. For those that are browning - wait, and just make sure they are in the right position relative to your lighting.
Over time you can start to add K ballance (if there are indicators of potassium deficiency and depending on which salt brand is used) with PIF. Leave a coupe of weeks between the introduction of each additional product - so you can evaluate its effect. PSG Iron etc can be added as indicators suggest.
Final phase, when all else is stable and sweet, Spur 2 maybe worth considering.
Zeo set out recomended start points for each product, but as Iwan says - he doses on the observation of coral condition which is an aquired skill, so all the more reason to phase products in gradulally. An added complication is that as stock increases, reactions can vary, so observation skills are needed.
Less is more

expect with food where more is more - and the power of the bacs at nutrient limitation shouldnt be underestimated so its hard to kick the parsimonious feeding habbit. I read somewhere that women are often better at this than men being more nutitionally aware.
HTH and keep us posted

There more of us using the Iwan hybrid system the more we can help each other out with the zeo tweaking skills.
Cheers
Simon